Fig. 5

DADS and luteolin treatment prevent neuronal death induced by overexpression of h4R-tau-GFP. a In pHuC-h4R-Tau-GFP-injected embryos, which respectively co-expressed Bcl2-L1 (panel a), Nrf2 (panel b), or GDNF (panel c), there are more neuronal cells survived. Statistical analysis (panel e) represents the quantitative results of zebrafish embryos respectively co-expressing Bcl2-L1 or Nrf2 or GDNF to have higher percentage of more neuronal cells compared to the control. The n value is indicated. b Numbers of zebrafish embryos with more neuronal cells were counted as described above for pHuC-h4R-Tau-GFP-injected embryos treated with DADS (diallyl-disulfide) (panel a) and luteolin (panel b). Statistical analysis (panel e) was presented similarly as described above to show that pHuC-h4R-Tau-GFP-injected embryos treated with DADS or luteolin have higher percentage of more neuronal cells compared to the control. c The effects of DADS and luteolin treatment on h4R-tau-GFP-induced neuronal death were confirmed by double immunostaining of GFP-labeled neurons at 48 hpf. The phosphorylation state of h4R-tau-GFP was detected by antibody pT212 (panels a and b) and antibody AT8 (panels a’ and b’). Embryos are shown from the lateral view with the anterior to the left and dorsal to the top. Scale bar: 50 μm