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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO

Fig. 2

Identification of the potential N-glycosylation sites of SR-AI. a and b COS 7 cells were transfected with human SR-AI, the single mutation of asparagine to glutamine at residues 82, 102, 143, 184, 221, 249 and 267. Western blot analysis was performed on the total cell lysates before (a) and after (b) treated by PNGase F, and avidin pull-down of biotinylated cell lysates (c). d and e COS-7 cells were transfected with hSR-AI and the double mutation of asparagine (N) to glutamine (Q) residues at 102-143, 102-184 and 143-184. Western blot analysis was performed on the total cell lysates (d) and the PNGase F or Endo H treated cell lysates (e). The result was repeated for three times (N = 3) and the representative blot was shown

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