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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO

Fig. 5

Critical residues in SRCR domain of human SR-AI for the surface targeting and N-glycosylation were identified by point mutation. COS-7 cells were transfected with wild type SR-AI and SR-AI variants. a Surface-targeted SR-AI and its variants were detected by live immunostaining (green). Cytosolic SR-A and its variants were detected by immunocytochemistry (red). Nuclei were counterstained with Hoechst 33258 (blue). b Cells were incubated with FAM-labeled oAβ. Representative merged confocal images of surface-targeted SR-AI (red) and internalized FAM-labeled oAβ (green) were shown. Nuclei were counterstained with Hoechst 33258 (blue). Scale bar, 10 μm. The result was repeated for four times (N = 4) and the representative image was shown

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