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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO

Fig. 6

Critical residues in the SRCR domain for the surface targeting and N-glycosylation of SR-AI and MARCO. a-c SR-AI variant G361P, E364A, S369S, L391A, F418A, F420A, G395A-Q397A, S424A-S425A, R432A, R437A, R4321A-R437A, H441R, and D444A were created by site-directed mutagenesis. Total cell lysates (A) and avidin pull-down of biotinylated lysates (B) were subjected to Western blot analysis. Western blot analysis of total cell lysates after PNGase F or Endo H cleavage (C). d-e, HEK293 cells were transfected with MARCO and variant R425A, R432A, G433A, R434A, E436A, W443A, V458A, F459A, C463A, L471A, V486A, H510A and D513A were created by site-directed mutagenesis. Total cell lysates (D) and avidin pull-down of biotinylated lysates (E) were subjected to Western blot analysis. The result was repeated for four times (N = 4) and the representative blot was shown

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