Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO

Table 1 Summary of the surface targeting and oAβ internalization of mutants in N-glycosylation sites of human SR-AI

Mutants	Complex glycan structure	Surface targeting	oAβ internalization
N82Q	✚	✚	✚
N102Q	↓	✚	✚
N143Q	↓	✚	✚
N184Q	↓	✚	✚
N221Q	✚	✚	✚
N249Q	✚	✚	✚
N267Q	✚	✚	✚
N102Q- N143Q	━	✚	↓
N102Q- N184Q	━	✚	✚
N143Q- N184Q	━	✚	↓

Complex glycan structure is able to be cleavage by PNGase F but not by Endo H; Hybrid and high mannose type glycans is able to be cleavage by both PNGase F and Endo H
✚, mutants are glycosylated in complex structure, surface-targeted or internalize normal amount of oAβ
━, mutants are not glycosylated in complex structure
↓, mutants are less glycosylated in complex structure or internalize less amount oAβ

ISSN: 1423-0127