Fig. 3

Influence of M3BIM on zebrafish embryonic development, and the ATP-release reaction and relative [Ca2+]i mobilization in human platelets. Wild-type zebrafish embryos (arrows) at 6 day postfertilization exposed to various concentrations of M3BIM (10, 20, 50, and 100 μM) or a solvent control (0.5 % DMSO) (scale bar 100 μm) (a,a). Statistical graphs (a,b) show the means ± SEM (n = 15). For other experiments, washed platelets (3.6 × 108 cells/mL) were preincubated with M3BIM (10, 20, 50, and 100 μM) or the solvent control (0.5 % DMSO), and 1 μg/mL of collagen was then added to stimulate either the ATP-release reaction (b) or to induce relative [Ca2+]i mobilization (c). Profiles (b and c) are representative of four independent experiments