Fig. 4

Regulatory effects of M3BIM on PLCγ2 and PKC activation in platelets. Washed platelets were preincubated with 25 or 50 μM M3BIM or a solvent control (0.5 % DMSO) and subsequently treated with 1 μg/mL of collagen or 150 nM PDBu to induce either PLCγ2 a or PKC activation (p47 phosphorylation) b and c and platelet aggregation d. Data are presented as the means ± SEM (n = 4). ** P < 0.01 and *** P < 0.001, compared with the resting platelets; # P < 0.05 and ## P < 0.01, compared with the platelets receiving the solvent control. Profiles d are representative of four independent experiments