Fig. 3From: CD44-mediated monocyte transmigration across Cryptococcus neoformans-infected brain microvascular endothelial cells is enhanced by HIV-1 gp41-I90 ectodomainCn and HIV-1 gp41-I90 co-induced adhesion and transmigration of THP-1 to HBMEC. a Induction of THP-1 adhesion with Cn and HIV-1 gp41 alone or Cn in combination with HIV-1 gp41. The adhesion of THP-1 was triggered by 5 × 106 CFU/ml of Cn, 2 μM of HIV-1 gp41-I90 or both of them. Joint use of Cn and HIV-1 gp41-I90 significantly induced THP-1 adhesion at 1.5 h after addition of THP-1 to the monolayer when compared with use Cn and HIV-1 gp41 alone. b Time-course study of Cn and HIV-1 gp41-I90 induced THP-1 transmigration across HBMEC monolayers. THP-1 transmigration was triggered by 5 × 106 CFU/ml Cn, 2 μM HIV-1 gp41-I90 or both of them. The values represent the mean of triplicate samples and are representative of one experiment from three independent experiments showing similar data. (*P < 0.05, **P < 0.01)Back to article page