Fig. 8

Redistribution of CD44 to membrane rafts during HBMEC exposure to Cn and/or HIV-1 gp41-190. HBMECs were treated with either PBS or Cn, or HIV gp41-I90 or both of them for 6 h. The cells were then lysed in buffer containing 1 % Triton X-100 on ice. Fractionation was performed in OptiPrep^TM gradients, and nine fractions were collected. The lipid raft fractions are indicated by * in fraction 2. An equal volume of each sample was analysed by dot blots using antibodies against CD44. Redistribution of CD44 on the membrane rafts was observed in Cn and/or HIV-1 gp41-I90 treated HBMECs