Fig. 5
Differentiated dopaminergic cells were subjected to patch clamp analysis. a When the cell was exposed to a current of 140pA for 10 ms, membrane potential was found polarized immediately and depolarized after application of the current. When exposed to a current of 70pA for 10 ms, the membrane potential of polarization and depolarization was observed with a delay. b Upon exposure to a current of 140pA for 10 ms, depolarization of cell membrane induced the opening of NaV channels, which in turn resulted in an influx of Na+ into the cells. Subsequently, opening of KV channels resulted in an efflux of K+ out of the cells. c After transplanting the cells into hemi-parkinsonian rats, a large number of cells were found to have survived in the host with positive staining for Tuj1 (neuron-specific class III beta-tubulin) at 4 weeks post-surgery. d After 6 weeks following transplantation, integration was clearly observable with the transplanted cells interspersed with the host cells. e Confocal microscopy showed that some cells have migrated across the host brain nucleus. f H&E staining of the host did not show any obvious teratoma formation. g Over 8 weeks, when tissues were extracted for electrophysiological studies of grafted cells, action potentials were induced when the cells were exposed to a current of 150 pA for 400 ms. Scale bars: C, D, E, 100 μm. F, 1 mm. Total number of rats transplanted with cells/control culture media is 6 for each group