Fig. 1
Stimulatory effect of resveratrol on TEA-sensitive potassium currents in embryonic rat cortical neurons. The whole-cell recording was conducted in these experiments. Because BKCa channels are typically blocked by externally applied tetraethylammonium (TEA, 1 mM), this compound is often used to determine the contribution of BKCa channels to the whole-cell currents. All cells were held at -50 mV in Tyrode’s solution containing 1.8 mM CaCl2. Then, cells were depolarized from −50 to +70 mV with a duration of 300 msec. a The currents were recorded in the presence of TEA (1 mM) combined with NS1619 (5 μM) from the same cell. b, c, d Original current traces are representative of 3 experiments. The currents were recorded in the presence of TEA (1 mM) or TEA (1 mM) combined with resveratrol (10, 20 and 40 μM) from the same cell. Resveratrol (20 and 40 μM, but not 10 μM) increased the TEA-sensitive current in these cells (n = 3). e Bar graph showing the effect of resveratrol on TEA-induced currents. Each bar indicates the mean ± SEM. (n = 3) *Significantly different from control (P < 0.01)