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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Betulinic acid synergically enhances BMP2-induced bone formation via stimulating Smad 1/5/8 and p38 pathways

Fig. 2

The effect of betulinic acid on BMP2-induced osteogenic differentiation and matrix mineralization in MC3T3-E1 preosteoblast cells. a RT-PCR analysis. The MC3T3-E1 cells were maintained with osteogenic medium containing BetA (10 μM) and/or BMP2 (200 ng/ml) for 10 days, and expression of osteogenic marker genes were measured by RT-PCR. b Mineralization assay. Cells were cultured as in (a), and were stained with alizarin red solution (upper panel). For quantification, the stains were eluted with 10 % cetylpyridinium and absorbance was measured by spectrophotometry (lower panel). *, p < 0.05 compared to the indicated group. Representative data are shown. n = 3

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