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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Betulinic acid synergically enhances BMP2-induced bone formation via stimulating Smad 1/5/8 and p38 pathways

Fig. 3

Effects of betulinic acid on phosphorylation of Smad, ERK and p38 in MC3T3-E1 cells. a Western blot analysis of phosphorylation of Smad, ERK and p38 before and after BetA (10 μM) and/or BMP2 (200 ng/ml) for 5 to 10 min. b to d Quantitative analysis. Phosphorylation of Smad1/5/8, ERK and p38 was quantified by using a LAS-4000 lumino-image analyzer system (Fujifilm, Japan). Each phosphorylation was normalized by the total amount of Smad1 (b), ERK (c) and p38 (d). *, p < 0.05 compared to the control group. #, p < 0.05 compared to the indicated group. Representative data are shown. n = 3

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Journal of Biomedical Science

ISSN: 1423-0127