Fig. 2

Mdivi-1 reduced Drp1 phosphorylation, protein oxidation, and DNA fragmentation after TGI. Rats were treated intraperitoneally with Drp1 inhibitor Mdivi-1 (2.4 mg/kg) or its solvent DMSO 30 min before TGI. Total proteins were extracted from the hippocampal CA1 subfield of sham-operated controls or treated animals 24 h after 10-min TGI for detection of p-Drp1(Ser616) in (a), protein oxidation in (b) and activated caspase-3 expression in (c). DNA was isolated from the hippocampal CA1 subfield of sham-operated controls, DMSO + I/R and Mdivi-1 + I/R 48 h after TGI for detection of DNA fragmentation by PCR assay (d), protein lysates from hippocampal CA1 tissues were collected 48 h after TGI for detection of DNA fragmentation by sandwich ELISA in (e). Values are mean ± SEM from representative blots and quantitative analysis from 5–6 animals in each experimental group (a, b and c). Values in (e) are fold changes with reference to sham-operated controls and are mean ± SEM of 4 animals in each experimental group. *P < 0.05 versus sham control group, #P < 0.05 versus DMSO + I/R group in the Scheffé multiple-range test. I/R: ischemia/reperfusion