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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Peroxisome proliferator-activated receptor-gamma dependent pathway reduces the phosphorylation of dynamin-related protein 1 and ameliorates hippocampal injury induced by global ischemia in rats

Fig. 4

Western blotting of p-Drp1(Ser616) and total Drp1 expression after Drp1-siRNA in the hippocampal CA1 subfield after TGI. After microinjection with Drp1-siRNA (0.05 nM in a total volume of 400 nl) into the CA1 subfield 24 h before TGI, total proteins were isolated from hippocampal CA1 subfield of sham-operated controls, control siRNA with TGI, or Drp1-siRNA animals after 10 min of TGI with 24 h reperfusion for detection of p-Drp1(Ser616) in (a) and total Drp1 in (b). The same blots were also probed with a α-tubulin antibody to serve as an internal control for equal loading of proteins in each lane. Values are mean ± SEM from representative blots and quantitative analysis from 4-6 animals in each experimental group. I/R: ischemia/reperfusion, NC: negative control siRNA

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