Fig. 6From: Peroxisome proliferator-activated receptor-gamma dependent pathway reduces the phosphorylation of dynamin-related protein 1 and ameliorates hippocampal injury induced by global ischemia in ratsDrp1 siRNA attenuates oxidative stress and decreases DNA fragmentation in hippocampal CA1 subfield after TGI. After microinjection with Drp1 siRNA (0.05 nM in a total volume of 400 nl) into the CA1 subfield 24 h before TGI, Total proteins were isolated from the hippocampal CA1 subfield of sham-operated controls, control siRNA with TGI, or Drp1 siRNA with TGI for protein oxidation in (a) and activated caspase-3 expression in (b). DNA was isolated from collected hippocampal CA1 subfield of sham-operated controls, vehicle with negative control siRNA, and Drp1-siRNA 48 h after TGI for detection of DNA fragmentation by PCR assay in (c) Hippocampal CA1 tissues were collected 48 h after TGI for detection of DNA fragmentation by sandwich ELISA in (d). Values are mean ± SEM from representative blots and quantitative analysis from 5–6 animals in each experimental group (a and b). Values are fold changes in (d) with reference to sham-control; mean ± SEM of 5–7 animals in each experimental group. *P < 0.05 vs. sham-control group and #P < 0.05 vs. negative control siRNA + I/R in the Scheff′e multiple-range test. I/R: ischemia/reperfusion, NC: negative control siRNABack to article page