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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Erinacine A-enriched Hericium erinaceus mycelium ameliorates Alzheimer’s disease-related pathologies in APPswe/PS1dE9 transgenic mice

Fig. 2

HE-Et and HE-My reduce amyloid plaque burden in the area include the cerebral cortex and hippocampus of APP/PS1 mice. Five month-old APP/PS1 mice were orally administered with vehicle (Veh, n = 6), HE-Et (n = 5) and HE-My (n = 6) for 30 days. A. The representative fluorescent images of amyloid plaques detected by thioflavin S (ThS) staining (white in a, e and i, and green in c, d, g, h, k and l) and immunohistochemical staining with AB-10 antibody (white in b, f and j, red in c, d, g, h, k and l) in the area including parietal cortex and hippocampus. Sale bar: 500 μm. A typical plaque is magnified and shows in the right side of each image. Sale bar: 20 μm. B. shows both the plaque number and burden in ThS- and AB-10-stained semi-cerebral sphere calculated by image analysis software. Plaque burden is displayed as a percentage of the area occupied by ThS- or AB-10-stained signal in the full area of interest. C. The structure of amyloid plaque in the cerebral cortex of APP/PS1 mice. The amyloid plaque in cerebral cortex of 6 months-old APP/PS1 mice was detected by ThS-staining (white in a and d, and green in c and f) and immunohistochemical staining with AB-10 antibody (white in b and e, and red in c and f). Upper panels show the representative Z-projection (3 dimensions, XYZ, A-C) of a typical plaque. Lower panels show the 2 dimensional images (XY, XZ, and YZ, D-F). The image indicated by arrow indicated the putative plaque unit. Sale bar: 10 μm. Single fluorescent images were presented as grayscale to enhance resolution

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