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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: WSB1: from homeostasis to hypoxia

Fig. 1

Regulation of WSB1 mRNA Expression by Hypoxia. Various cell lines including HeLa, Hep3B, RD, Daoy, and HMEC were treated with severe hypoxia for various time points (6, 12 and 24 h) as per methods published previously [34] and total mRNA extracted and evaluated by expression microarray. Panel a shows the normalized Log2(Treated/Untreated) ratios of EST AL110269 (wsb1) plotted for each time point. Cell lines are indicated by differing colors/lines. Panel b shows the normalized Log2(Treated/Untreated) ratios of EST AB018285 (JHDM2A/JMJD1A), a known HIF-1 target [31, 46] plotted for each time point. mRNA levels at Time0 are normalized to the Log2 value “0”. Cell lines are indicated by differing colors/lines. For both panels, Hela cells treated with 260uM desferroximine (Hela-DFX) was used as a positive control

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