Fig. 5
Characterization of WSB1 Induction with Doxycycline. Panel a HEK293 cells containing the inducible WSB1 “C” construct (293-C2) or native HEK293 cells (293) were treated with various concentrations of doxycycline (250, 500, 1000, 2000 ug/ml) for 24 h, then used for Western blotting with #604 anti-WSB1 and anti-pTEN (New England Biolabs) primary antibodies followed by detection with anti-rabbit Ig secondary antibody. Lysates from Hela cells treated with desferroximine (260 uM) were used as positive controls (WSB-1 (+)). Panel b HEK293 cells containing the inducible WSB1 “C” construct (293-C2) were treated with 1000 ug/ml doxycycline for various time points (in hours) and then subjected to Western blotting as for Panel A (above). Lysates from Hela cells treated with desferroximine (260 uM) were used as positive controls (WSB-1 (+))