Fig. 1

thsd7a was first expressed in primary motor neurons. Whole-mount ISH showed the spatiotemporal expression pattern of thsd7a, islet I and islet II at 24 hpf in wild type zebrafish embryos. Anterior is to the left. a: thsd7a transcripts were detected along the ventral edge of the neuron tube indicated by arrowheads. b, c: islet I (MiP and RoP marker) and islet II (CaP marker) were expressed in primary motor neurons along the ventral edge of the neuron tube indicated by arrowheads. Rohon-Beard sensory neurons can be seen along the dorsal edge of the neuron tube. a’, b’, c’: Enlarged images of the boxed regions in panel a, b and c, respectively. Scale bar is 100 μm