Fig. 2

The signals of Tg(thsd7a:GFP) were detected in primary motor neurons. Representative images of the Tg(thsd7a:GFP) transgenic zebrafish at 48 hpf. Thsd7a and motor neurons were shown in green and red, respectively. Anterior is to the left. a-c GFP signals driven by thsd7a promoter were detected in the brain and the neural tube, consistent with ISH results. Moreover, the signals at the neural tube co-localized with anti-Znp1 antibody (shown in red). d-f Enlarged images of the neural tube showed GFP signals were expressed in primary motor neurons, indicated by arrows