Fig. 4

Knockdown of Thsd7a affected expression of genes related to Notch/dll4 signaling pathway. MO1 and 5msMO1 treated embryos at 48 hpf were collected for quantitative real-time PCR. thsd7a expression was down-regulated by 49 %. Notch1b was significantly downregulated by Thsd7a knockdown. A 51 % decrease of notch1b expression was observed in the Thsd7a morphants as compared with 5msMO1. Co-injection of MO1 and thsd7a mRNA increased both thsd7a and notch1b expression levels by 24 %. Endogenous β-actin was used as an internal control. Each experiment was performed in triplicate and repeated at least three times. *, P < 0.05; **, P < 0.01; Student’s t-test. The error bars shown mean ± SEM