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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Glycine N-methyltransferase deficiency in female mice impairs insulin signaling and promotes gluconeogenesis by modulating the PI3K/Akt pathway in the liver

Fig. 5

GNMT deficiency impaired insulin-stimulated Akt and S6 ribosomal protein activation in the liver. Representative Western blot analysis and quantification of expression and insulin-stimulated phosphorylation of Akt, S6 ribosomal protein, and IR in the liver of male a and female b WT and Gnmt−/− mice (n = 4–5 per group). α-Tubulin was used as a loading control. The levels of immunoreactive phosphorylated proteins were normalized to the total expression of the respective protein. All error bars indicate s.e.m. ** p < 0.01 (between baseline and insulin treatment); * p < 0.05 (between baseline and insulin treatment); # p < 0.05 (between insulin-treated WT and Gnmt−/− mice)

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