Fig. 2

Proliferation against rhCEA by PBMCs collected before, during, and after DC vaccination from different patients. PBMCs collected before (day 0), during, and after DC vaccination (days 36, 57, 120, and 180) were cultured in 96-well culture plates for 5 days in the presence of 0 (PBS), 5, 10, 25, or 50 μg/mL rhCEA. Cellular proliferation was determined using a BrdU incorporation ELISA. T cell proliferation status was expressed as the OD value measured at 450 nm (reference wavelength 650 nm). The OD value obtained from culture with different concentrations of rhCEA was compared to that of culture with PBS control. *p < 0.05, **p < 0.001