Fig. 3
From: Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells
Induction of oxidative stress by E2. Reactive oxidative species (ROS) formation in ACHN cells were detected by flow cytometry (28 μM E2, orange line). DCFH-DA-pretreated cells as ROS-positive were incubated with 50 μM DCFH-DA for 30 min at 37 °C. Blue line, 0.1 % DMSO (negative control); red line, 1 mM H2O2 (positive control). E2, 17-β-estradiol