Fig. 4From: Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cellsPhosphorylation of nuclear Nrf2 by E2. Cytoplasm and nuclear extracts were respectively isolated from ACHN cells. Each compartment was immunodetected with antibody raised against phospho Nrf2 (Ser40), lamin A/C (for nuclear control), and α-tubulin (for cytosol control). Each protein level relative to individual control was determined by densitometry and shown on the bottom of each band. E2, 17-β-estradiolBack to article page