Fig. 5

Results from immunoblot (IB) using an antiserum against SUMO-1 on proteins immunoprecipitated (IP) by an anti-IkBα antiserum from the RVLM (a); western blot analysis of expression of IkBα relative to β-actin in the RVLM (b); transcriptional activity of NF-kB p65 in the RVLM (c) after intravenous administration of saline or LPS (10 mg kg⁻¹); or animals that received pretreatment by microinjection bilaterally into RVLM of NRS (1:20), anti-SUMO-1 antiserum (SUMO-1 Ab; 1:20) or anti-Ubc9 antiserum (Ubc9 Ab; 1:20) during Phase III in the experimental endotoxemia model of brain death. Values are mean ± SEM of duplicate analyses on individual samples obtained from 5–6 animals per experimental group. *P < 0.05 versus corresponding saline group and ⁺ P < 0.05 versus NRS + LPS group in the post hoc Scheffé multiple-range test