Fig. 6
Representative western blots (insets), or percentage of NOS II or nitrotyrosine (NT) relative to β-actin in the RVLM detected after intravenous administration of LPS (10 mg kg−1) in animals (a); or animals that received pretreatment by microinjection bilaterally into the RVLM of NRS (1:20), anti-SUMO-1 antiserum (SUMO-1 Ab; 1:20) or anti-Ubc9 antiserum (Ubc9 Ab; 1:20) during phase III in the experimental endotoxemia model of brain death (b). Values are mean ± SEM of duplicate analyses on individual samples obtained from 5–6 animals per experimental group. *P < 0.05 versus corresponding saline group in the post hoc Scheffé multiple-range test. Note that in (a), dividing lines are placed on the representative gel images to denote re-grouping of images from different parts of the same gel