Fig. 4

Heme oxygenase-1 expression, serum ASAT, ALAT levels, glycogen and total fat content in the livers. C57BL/6 N mice were treated either with LPS (5 mg/kg body weight), AMD3100 (5 mg/kg body weight), with both substances or with the solvent PBS (control). 24 h after LPS administration, the mice were sacrificed and blood and livers were collected for further biochemical and histological analysis. (a-i): Representative photomicrographs from one of seven different tissue samples stained for HO-1 expression are shown (immunohistochemistry (red-brown color), counterstaining with hematoxylin, original magnification: (a-d, f-i) 400×, (c) 630×). The photomicrograph in (e) shows a single Kupffer cell at a higher magnification (630×). As an approximate measurement for the fat content, we determined the turbidity value in the 9000 g supernatants of the livers (l). j and k depict the serum concentrations of ASAT and ALAT of all treatment groups. Data are given as mean ± standard error of the mean (SEM); n = 7 for each group. Statistical significant differences between the different treatment groups were determined by using the one-way analysis of variance (ANOVA) and the Tukey post hoc test and are indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. control animals; ⁺, p < 0.05; ⁺⁺, p < 0.01; ⁺⁺⁺, p < 0.001 vs. LPS treatment; #, p < 0.05; ##, p < 0.01; ###, p < 0.001 vs. AMD3100 administration