Table 1 Primary antibodies used for immunohistochemistry (IHC) and immunoblotting (IB)
Primary antibody | Type, Catalogue number | Manufacturer | Dilution IHC/IB | Host species |
|---|---|---|---|---|
CD3 | polyclonal, sc-20047 | Santa Cruz Biotechnology | 1:500/1:200 | Mouse |
CD8 | polyclonal, sc-7188 | Santa Cruz Biotechnology | 1:200/- | Rabbit |
CD68/ED1 | monoclonal, MCA341R | AbDSerotec | 1:50/- | Mouse |
cleaved caspase-3 | monoclonal, 9661 | Cell Signaling Technology | 1:600/1:1000 | Rabbit |
CYP3A | polyclonal | Daiichi Pure Chemicals | 1:5000/1:10000 | Goat |
CYP2B | polyclonal | Daiichi Pure Chemicals | 1:5000/1:10000 | Goat |
CYP2E1 | polyclonal | Daiichi Pure Chemicals | 1:5000/1:10000 | Goat |
heme oxygenase 1 | polyclonal, SPA-895 | Biomol GmbH | 1:5000/1:10000 | Rabbit |
iNOS | polyclonal, sc-651 | Santa Cruz Biotechnology | 1:500/- | Rabbit |
Nrf-2 | polyclonal, sc-722 | Santa Cruz Biotechnology | -/1:500 | Rabbit |
TNF alpha | monoclonal, sc-52746 | Santa Cruz Biotechnology | 1:500/- | Mouse |
VEGF | monoclonal, sc-7269 | Santa Cruz Biotechnology | 1:500/- | Mouse |