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Table 1 Primary antibodies used for immunohistochemistry (IHC) and immunoblotting (IB)

From: Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo

Primary antibody Type, Catalogue number Manufacturer Dilution IHC/IB Host species
CD3 polyclonal, sc-20047 Santa Cruz Biotechnology 1:500/1:200 Mouse
CD8 polyclonal, sc-7188 Santa Cruz Biotechnology 1:200/- Rabbit
CD68/ED1 monoclonal, MCA341R AbDSerotec 1:50/- Mouse
cleaved caspase-3 monoclonal, 9661 Cell Signaling Technology 1:600/1:1000 Rabbit
CYP3A polyclonal Daiichi Pure Chemicals 1:5000/1:10000 Goat
CYP2B polyclonal Daiichi Pure Chemicals 1:5000/1:10000 Goat
CYP2E1 polyclonal Daiichi Pure Chemicals 1:5000/1:10000 Goat
heme oxygenase 1 polyclonal, SPA-895 Biomol GmbH 1:5000/1:10000 Rabbit
iNOS polyclonal, sc-651 Santa Cruz Biotechnology 1:500/- Rabbit
Nrf-2 polyclonal, sc-722 Santa Cruz Biotechnology -/1:500 Rabbit
TNF alpha monoclonal, sc-52746 Santa Cruz Biotechnology 1:500/- Mouse
VEGF monoclonal, sc-7269 Santa Cruz Biotechnology 1:500/- Mouse