Fig. 1
From: Therapeutic effect of berberine on TDP-43-related pathogenesis in FTLD and ALS
The effect of berberine on insoluble TDP-43 accumulation. a and b After 6Â h (a) and 24Â h (b) transfection, N2a cells were transfected with GFP-tagged aggregation-prone TDP-43 fragments (GFP-TDP-25) and treated with different dose of berberine for another 24Â h. The soluble/insoluble TDP-43 were extracted by RIPA/urea fractionation and analyzed by Western blotting with anti-GFP antibody