Fig. 4

The Notch ligand Jagged1 is a functional YAP1 target. a and b High expression of miR-199a-3p and YAP1 silencing significantly inhibit protein level of Jagged1. c and g Wetern blot assays confirm that transfection is successful for all combinations. d–f Overexpression of miR-199a-3p reduces cell proliferation and induces apoptosis, however, Jagged1 relieves these effects in Huh7 cells. h–j si-YAP1 significantly inhibits cell proliferation and induces apoptosis in Huh7 cells, while Jagged1 reverses these effects. *P < 0.05, **P < 0.01 and ***P < 0.001