Fig. 4
From: Suberoylanilide hydroxamic acid represses glioma stem-like cells
SAHA causes cell cycle arrest of GSCs. a GSCs from U87MG cells were treated with SAHA (1 μM and 2.5 μM) for 24 h. The spheres were harvested for PI staining and analyzed by using flow cytometry. b Data regarding the population of sub-G1, G1, S, G2/M and > 4 N upon SAHA treatment was presented as the mean ± S.D. of experiments performed in triplicate; bar, S.D.; *p < 0.05, **p < 0.01. c The expression levels of cyclin B1, CDK1, PCNA, and p21 were measured by Western blotting. Tubulin was used as a loading control