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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells

Fig. 1

CD271+ OS cells have higher autophagy level than CD271- OS cells under LH condition. a CD271+ and CD271- OS cells were separated by MACS. Then, the percentage of CD271+ cells in the indicated OS cells was detected with FACS assay. The data are shown as the mean ± S.D. (n = 3; **, P < 0.01). b The mRNA expression of autophagy-relative genes in the CD271- and CD271+ SaoS2 cells under normal and LH conditions at 24 h was examined with qPCR. The data are shown as the mean ± S.D. (n = 3; **, P < 0.01). c The protein level of LC3 and p62 in the indicated OS cells under normal and LH conditions at 24 h was detected by western blotting. d The GFP-LC3-transfected OS cells under normal and LH conditions at 24 h were captured with a fluorescence microscope. The mean number of GFP-LC3 dots per the indicated OS cells from three replicates are shown as the mean ± S.D. (**, P < 0.01)

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