Fig. 2
From: Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells
Autophagy-deficiency has no significant influence on basic biological activities in OS cells under normal condition. a–i The indicated OS cells were cultured in normal condition. a, f The protein level of Atg5 and Atg7 in the transfected SaoS2 (a) and MNNG/HOS (f) cells was detected by western blotting. b, g The percentage of CD271+ cells in the transfected SaoS2 (b) and MNNG/HOS (g) cells were examined with FACS assay. The data are shown as the mean ± S.D. (n = 3). c The cell viability of the indicated SaoS2 cells was detected with CCK8 assay at the indicated time points. The data are shown as the mean ± S.D. (n = 3). d, h The indicated SaoS2 cells were stained with BrdU and images were captured with a fluorescence microscope. The percentage of Brdu + cells in the indicated SaoS2 (d) and MNNG/HOS (H) cells was shown in graphs. The data from three replicates are shown as the mean ± S.D. e, i The cell death of the indicated SaoS2 (e) and MNNG/HOS (i) cells was analyzed by Annexin-V-FITC/PI double staining with FACS. The percentage of dead cells (Annexin-V-positive or PI-positive) was shown in graph. The data from three replicates are shown as the mean ± S.D