Fig. 3
From: Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells
Autophagy-deficiency reduces the advantages of CD271+ OS cells in terms of resistance to LH condition. a–h The indicated OS cells were cultured in LH condition. a, f The cell viability of the indicated SaoS2 (a) and MNNG/HOS (f) cells was detected by CCK8 assay at the indicated time points. The data are shown as the mean ± S.D. (n = 3; *, P < 0.05; **, P < 0.01). b, g The LDH release level of the indicated SaoS2 (b) and MNNG/HOS (g) cells under LH treatment was detected at 24 h. The data are shown as the mean ± S.D. (n = 3; *, P < 0.05; **, P < 0.01). c The caspase-3 activity of the indicated SaoS2 cells under LH treatment was detected at 24 h. The data are shown as the mean ± S.D. (n = 3; *, P < 0.05; **, P < 0.01). d, h The cell death of the indicated SaoS2 (d) and MNNG/HOS (H) cells under LH treatment was analyzed by Annexin-V-FITC/PI double staining with FACS. The percentage of dead cells (Annexin-V-positive or PI-positive) was shown in graph. The data from three replicates are shown as the mean ± S.D. e The percentage of CD271+ cells in the transfected SaoS2 cells under LH treatment at the indicated times was examined with FACS assay. The data are shown as the mean ± S.D. (n = 3)