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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells

Fig. 4

Autophagy-deficiency eliminates the advantages of CD271+ OS cells in terms of resistance to chemotherapeutics. a, b The indicated SaoS2 and MNNG/HOS cells were treated with Cisplatin or Epicubicin of different doses for 48 h, and IC50 of the indicated OS cells to Cisplatin (a) or Epicubicin (b) were evaluated by CCK8. The data are shown as the mean ± S.D. (n = 3; **, P < 0.01). c The LDH release level of the indicated SaoS2 and MNNG/HOS cells was detected at 24 h after Cisplatin (4 μg/ml) treatment. The data are shown as the mean ± S.D. (n = 3; *, P < 0.05; **, P < 0.01). d The caspase-3 activity of the indicated SaoS2 and MNNG/HOS cells was detected at 24 h after Epirubicin (2 μg/ml) treatment. The data are shown as the mean ± S.D. (n = 3; *, P < 0.05; **, P < 0.01). e, f FACS assay detected the percentage of CD271+ cells in the indicated SaoS2 and MNNG/HOS cells at 24 h after Cisplatin (4 μg/ml, e) or Epirubicin (2 μg/ml, f) treatment. The data are shown as the mean ± S.D. (n = 3; *, P < 0.05; **, P < 0.01)

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