Fig. 5
From: Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells
Autophagy-deficiency decreases the advantage of CD271+ OS cells in terms of tumorigenesis in vitro. a–d The colony-forming and sphere-forming abilities of the indicated SaoS2 (a, b) and MNNG/HOS (c, d) cells were detected. The mean number of colonies (a, c) and spheres (b, d) per well and the mean volume of spheres in the indicated groups are shown in graphs. The data from three replicates are shown as the mean ± S.D. e The protein expression of Nanog, Oct3/4, Stat3 and p-Stat3 in the indicated OS cells were examined by western blotting