Fig. 4
From: Loss of porin function in dopaminergic neurons of Drosophila is suppressed by Buffy
Inhibition of porin in the developing eye results in phenotypes that may be suppressed by Buffy and enhanced by α-synuclein. Scanning electron micrographs when porin is inhibited in the developing eye and co-expressed along with either Buffy or α-synuclein. The genotypes are (i) GMR-Gal4/ UAS-lacZ, (ii) GMR-Gal4/ UAS-porin-RNAi (1), (iii) GMR-Gal4/ UAS-porin-RNAi (2), (iv) UAS-Buffy; GMR-Gal4/ UAS-lacZ, (v) UAS-Buffy; GMR-Gal4/ UAS-porin-RNAi (1), (vi) UAS-Buffy; GMR-Gal4/UAS-porin-RNAi (2), (vii) UAS-α-synuclein; GMR-Gal4/UAS-lacZ, (viii) UAS-α-synuclein; GMR-Gal4/ UAS-porin-RNAi (1), and (ix) UAS-α-synuclein; GMR-Gal4/UAS-porin-RNAi (2). Biometric analysis when (x) porin is inhibited in the eye indicated decreased ommatidia number and higher percentage of ommatidial disruption when compared to the control. (xi) The overexpression of Buffy with porin-RNAi results in restoration of the number of ommatidia and the degree of ommatidial disruption to below the control levels. (xii) The inhibition of porin along with α-synuclein expression resulted in the enhancement of the eye phenotypes when compared to controls as displayed by the low number of ommatidia coupled by the high degree of disruption of the ommatidial array. All comparisons were determined by a one-way analysis of variance followed by a Dunnett’s multiple comparison test (P < 0.05), error bars are SEM, asterisks (*) represent statistical significance and n = 10