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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Differential humoral and cellular immunity induced by vaccination using plasmid DNA and protein recombinant expressing the NS3 protein of dengue virus type 3

Fig. 1

Schematic representation of the immunization schedule. Groups of BALB/c mice (n = 4) received three doses of GST-NS3-DEN3 or GST by the intraperitoneal (i.p.) route with one week of interval between each dose, or two doses of pcDNA/NS3-DEN3 or pcDNA by the intramuscular (i.m.) route with two weeks of interval between each dose. Two weeks after the third and second dose respectively, spleen and antiserum samples were collected from these animals. Splenocytes obtained were used for proliferation assays and determination of cytokines by RT-PCR. Moreover, the immune serum is used for determination of cytokines by ELISA and to analyze the antibody titer anti-NS3. To control the experiment, the same procedure was performed in parallel with splenocytes collected from non-vaccinated mice (n = 4)

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Journal of Biomedical Science

ISSN: 1423-0127