Fig. 3

Splenic cell proliferation upon stimulation with NS3-DEN3. The MTT assay was performed with splenocytes from normal mice or pcDNA3, pcDNA3/NS3DEN3, and NS3DEN3 immunized mice (cells collected 7 days after the last immunization, three times at one-week intervals). Non-stimulated (NS) (White bars) or stimulated with Con A (checkered bars), NS3DEN3 10 μg/mL (Black bars), or NS3DEN3 50 μg/mL (striped bars). (***P < 0 · 0001)