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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Differential humoral and cellular immunity induced by vaccination using plasmid DNA and protein recombinant expressing the NS3 protein of dengue virus type 3

Fig. 4

Effect of immunization with pcDNA3/NS3DEN3 and recombinant NS3DEN3 protein on cytokine gene expression in splenocytes of BALB/c mice. a RT-PCR analysis of cytokines mRNA in splenocytes (a) Normal mice, (b) mice immunized with pcDNA3, (c) mice immunized with pcDNA3/NS3DEN3, and d) mice immunized with NS3DEN3. (Lane 1) non-stimulated cells, or stimulated (Lane 2) Con A, (Lane 3) NS3DEN3 10 μg/mL, (Lane 4) NS3DEN3 50 μg/mL. GAPDH was used as control housekeeping gene. b Profile of cytokines induced by NS3DEN3 in splenocytes. Splenocytes from immunized mice and normal mice were stimulated with the NS3DEN3 protein for 120 h, and cytokines was measured in cells culture supernatants by ELISA. IL-1 (open bars), IL-6 (full bars), and TNFα (checkered bars). Histograms show values in pg/mL (means ± SD) of three experiments run in duplicate. *,**,***P < 0.05, 0.001, and 0.0001, respectively, versus unstimulated cells

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Journal of Biomedical Science

ISSN: 1423-0127