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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Differential humoral and cellular immunity induced by vaccination using plasmid DNA and protein recombinant expressing the NS3 protein of dengue virus type 3

Fig. 5

Analysis of antibodies against NS3. a Determination of anti-NS3 antibody titer. NS3DEN3 protein was applied to a 96-well ELISA plate, and anti-NS3 antibodies at different dilutions were added in different wells. Full bars denote the antibody titer obtained by immunization with the recombinant protein. Open bars denote the antibody titer obtained by immunization with plasmid pcDNA3/NS3DEN3. Pre-immune serum was used as a negative control. Bound antibody was determined using secondary antibody tagged with HRP. Each bar represents the mean ± standard error of the mean from three independent experiments. b Analysis of antibodies against NS3 by Western blotting. NS3DEN3 was run in SDS-PAGE, transferred to nitrocellulose membrane. The membrane was cut into strips, and each strip was developed with primary antibodies at different dilutions followed by alkaline phosphatase-conjugated secondary antibodies. (a) Serum obtained from mice immunized with the recombinant protein, and (b) serum obtained from mice immunized with DNA. Lane 1, 1:100; lane 2, 1:200; lane 3, 1:400; lane 4, 1:800; lane 5, 1:1600 and lane 6, 1:3200. A representative blot from three independent experiments is presented here

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Journal of Biomedical Science

ISSN: 1423-0127