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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Aberrant methylation of DACT1 and DACT2 are associated with tumor progression and poor prognosis in esophageal squamous cell carcinoma

Fig. 1

The mRNA expression and methylation analysis of DACT1, DACT2 and DACT3 in esophageal cancer cell lines. a, b, c Schematic structure of CpG islands in DACT1, DACT2 and DACT3 genes. The MSP and BGS-regions were shown in the schematic. The transcription start point was as +1. d The changes of DACT1, 2, 3 mRNA expression in treated or untreated cells with 5-Aza-Dc or TSA by RT-PCR method; e, f, g Relative mRNA expression of DACT1, 2, 3 in treated or untreated cells with 5-Aza-Dc or TSA by Quantitative realtime RT-PCR method. h The methylation status of DACT1, 2, 3 in four cell lines with(+) or without(−) 5-Aza-Dc treatment detected by MSP method. M, methylated gene; U, unmethylated gene. i The methylation status of every CpG site in two regions of DACT1, one region of DACT2 and one region of DACT3 in four tumor cell lines by BGS method. The color of circles for each CpG site represents the percentage of methylation. The “F” and “R” represent forward and reverse primers for MSP, respectively

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