Fig. 3

The expression and methylation analysis of DACT1, DACT2 and DACT3 in ESCC and corresponding adjacent non-cancerous tissues. a Representative RT-PCR results of DACT1, 2, 3 in 5 matched pairs (case1-case5) of ESCC tissues (T) and non-cancerous tissues (N). GAPDH was used as an endogenous control. b Relative mRNA expression of DACT1, 2, 3 in ESCC and adjacent tissues. c Representative immunohistochemical staining of DACT1, 2, 3 in ESCC tumor tissues (SP × 200). a: the positive expression of DACT1; b: the positive expression of DACT2; c: the positive expression of DACT3; d Positive expression frequency of DACT1, 2, 3 proteins in ESCC and corresponding adjacent tissues. e, f Relative mRNA expression of DACT1 and DACT2 in different subgroups of ESCC cases, which was expressed as the mean ± standard deviation. ^* P < 0.05, ^** P < 0.01. g Representative MSP results of DACT1, DACT2 and DACT3 in 3 matched pairs (case1-case3) of tumor tissue (T) and non-cancerous tissues (N). M, Methylation; U, Unmethylation; PC, positive control; NC, negative control. h The methylation rate of DACT1, 2, 3 in ESCC tissues and non-cancerous tissues. ^** P < 0.01. i The relative mRNA expression of DACT1 and DACT2 in methylated or unmethylated groups. The mRNA expression of DACT1 and DACT2 were all associated with methylation status of their TSS-regions in ESCC. ^** P < 0.01