Fig. 2

Apigenin inhibits the expression of EBV lytic proteins in EBV-positive Cells. (a) Epithelial NA cells were tested to detect the expression of EBV lytic proteins. Left panel: for detection of enhancement of reactivation, various concentrations of apigenin were added to the cells for 25 h, and then cell lysates were collected for western blotting. Right panel: for detection of inhibition of reactivation, the cells were pre-treated with apigenin for 1 h, then TPA + SB co-treatment was used for EBV induction. After 24 h of incubation, the cell lysates were analyzed by western blotting with antibodies against EBV Zta, Rta, EAD, DNase and β-actin. (b) Epithelial HA cells and (c) Burkitt’s lymphoma P3HR1 cells were tested for detection of inhibition of EBV reactivation using a similar procedure