Fig. 5

Apigenin represses Zp and Rp activities under different inductions. (a) Control plasmid PGL2, Zp, or Rp was transfected into NA cells. After 3 ~ 4 h of transfection, apigenin was added or not for pre-treatment for 1 h, and then TPA + SB were used to induce EBV into the lytic cycle. After induction for a further 24 h, lysates were collected for measurement of luciferase activity. Zp or Rp were co-transfected with (b) Zta-expressing and (c) Rta-expressing plasmids into NA cells for 3 h, then apigenin was added. After 24 h of transfection, luciferase activity was detected in the cell lysates. (d) Zp or Rp plasmid was transfected into TW01 cells. After 3 ~ 4 h of transfection, apigenin was added or not for pre-treatment for 1 h, and then TPA + SB was used to induce EBV reactivation. After induction for 24 h, cells lysates were produced for measurement of luciferase activity. Zp or Rp were co-transfected into TW01 cells with (e) Zta-expressing or (f) Rta-expressing plasmids. After 24 h of transfection, luciferase activity was detected in the cell lysates. The mean and standard deviation of each sample were calculated based on duplicates from three independent experiments