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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Inhibition of Epstein-Barr virus reactivation by the flavonoid apigenin

Fig. 6

Identification of the response element of Zp required for apigenin inhibition of EBV. (a) A schematic diagram of the −221 to +1 region of Zp that drives the luciferase gene in the reporter plasmid (left panel). For the analysis of activities of Zp deletion mutants in response to apigenin plus TPA + SB induction. TW01 cells were transfected with these deletion mutants. Three hours after transfection, the cells were pre-treated with apigenin for 1 h and TPA + SB were added to activate EBV (right panel). The relative luciferase activities were calculated as described in Methods. (b) A schematic diagram of the indicated domain structure and cellular factor binding sites on Zp (−221 to +1). The relevant mutated sites are indicated by black triangle (▲) on the diagram (upper panel). For analysis of luciferase activities of Zp mutants upon apigenin plus TPA + SB treatment, TW01 cells were transfected with the mutants indicated for 3 h and pre-treated with apigenin, then TPA + SB were added for 24 h to activate EBV (lower panel). The luciferase activities were detected and were calculated as described in Methods. The mean and standard deviation of each sample were calculated in duplicate from at least two independent experiments

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