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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Oxidized LDL-induced JAB1 influences NF-κB independent inflammatory signaling in human macrophages during foam cell formation

Fig. 3

Knock-down of JAB1 mRNA and protein level in human MФ. PMA-differentiated human U937 MΦ were transfected with siRNA JAB1 (siJAB1-MФ) or negative siRNA (nsiJAB1-MФ) for 48 h. Non-transfected MФ, nsiJAB1-MФ and siJAB1- MФ were treated with or without (control) oxLDL (50 μg/ml) for 4 h. a The JAB1 mRNA expression levels were determined using RT-qPCR. Bars represent mean ± SEM of 3 independent experiments. *p < 0.05 significance vs. non-transfected MФ or nsiJAB1-MФ. b The JAB1 and α-tubulin protein levels were determined using western blot analysis and quantified by ImageJ. Expression was normalized against α-tubulin. Bars represent mean ± SEM of 5 independent experiments. [m] – non-transfected MФ, [si] - siJAB1-MФ, [nsi] – nsiJAB1-MФ; *p < 0.05 significance vs. non-transfected MФ or nsiJAB1-MФ, # p < 0.05 significance vs. control

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