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Figure 2 | Journal of Biomedical Science

Figure 2

From: Marrow adipocytes inhibit the differentiation of mesenchymal stem cells into osteoblasts via suppressing BMP-signaling

Figure 2

CM-Adipo exerts paracrine inhibitory effect on osteoblast differentiation of mBMSCs. a Dose dependent inhibitory effect of the CM-Adipo on the osteoblast differentiation of mBMSCs as measured by quantitative alkaline phosphatase activity (ALP) after 6 days of osteogenic induction and (b) Alizarin red staining for matrix mineralization after 12 days of induction. Representative images of the ALP activity and ALZ red staining were shown at 100% concentration of the CM. c Downregulated osteogenic genes in mBMSCs when cultured in CM-Adipo versus CM-Control after 6 days of osteogenic induction. Gene expression was measured by qPCR using osteogenic RT2 profiler array as described in M&M. Values are mean ± SD of three independent experiments, (*p < 0.05, **p < 0.005)

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